Detection Technologies for Mines and Minelike Targets: 17-21 by Abinash C. Dubey, Ivan Cindrich, Society of Photo-Optical

By Abinash C. Dubey, Ivan Cindrich, Society of Photo-Optical Instrumentation Engineers

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J. E .. in: Methods in Enzymology. Vol. III (S. P. Colowick and N. O. ). Academic Press. New York (1957). p. 397. 8. Rose. I. • Kellermeyer, R .. Stjernholrn. R.. and Wood. H. G .. J. Bioi. Chern. 237:3325 (1962). S. Abraham 59 9. Matthes, K. J .. Abraham. S.. and Chaikoff, I. L.. Biochim. Biophys. Acta 70:242 (1963). 10. , Ludowieg. H •• Normore. • Biochemistry 2:1009 (1963). 11. San Pietro, A.. J. BioI. Chern. 217:579 (1955). 12. Horecker, B. L .. , J. Bioi. Chern. 175:385 (1948). 13. K .. • J.

It is the purpose of the present report to mention some of the compounds that have been recently prepared by biological methods and to describe, in some detail, the synthesis of glucose-3,4-C14. The preparation of this hexose with C14 in carbons 3 and 4 by conventional organic chemical methods would, of course, be extremely difficult. METHODS MAKING USE OF PURIFIED ENZYME SYSTEMS Conversion of Glucose to Fructose by Organic and Biological Procedures One of the most successful methods for the conversion of isotopically-labeled glucose into isotopically-labeled fructose has been the procedure devised by Muntz and Carroll [1) in yields which exceed 70%.

34 Table XII. 2 B. E. Gordon and J. J. Madison 37 Table XIII. 47 Table XIV. 9 "/0 H replaced by D Methods for Labeling Compounds 38 Table XV. 7 0 1 2 3 4 5 6 7 8 9 10 11 Table XVI. Deuterium Distribution, Run 15-Dz/n-C 5 Number of D atoms per molecule 0 1 2 3 4 5 6 7 8 9 10 11 12 "/0 H replaced by D Hydrocarbon. "/om. 2 C14-LABELING USING CARBENE INSERTIONAPPLICATION TO SATURATED HYDROCARBONS * M. A. Muhs, E. L. Bastin, and B. E. , less use is made of them than would be expected. This is due chiefly to difficulties in obtaining radioactive tracers in their proper chemical forms.

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