Advances in Tracer Methodology: Volume 2 A collection of by Martin D. Kamen (auth.), Seymour Rothchild (eds.)

By Martin D. Kamen (auth.), Seymour Rothchild (eds.)

Advances in Tracer method, quantity 2, documents the professional­ ceedings of the sixth, seventh, and eighth Symposia on Advances in Tracer method. those Symposia, that are a part of a continual sequence subsidized by means of the hot England Nuclear Corp. and the Packard device corporation, Inc., are dedicated to the total isotope tracer box: coaching and research of categorised compounds, functions within the chemical, biochemical, and medical fields, and well-being physics issues linked to tracer paintings. The papers during this quantity mirror definite tendencies which might be famous within the tracer box: expanding reliance on biochemical tools for labeling esoteric compounds, growing to be understanding of the issues of radiation decomposition, using complex instrumentation for the research and detection of radioactive substrates, and the swiftly transforming into program of tracer compounds to scientific prognosis. the 3 Symposia at which the papers have been awarded have been hung on the subsequent dates: DATE situation CHAIRMAN 11/16/62 manhattan urban Daniel Steinberg, leader Laboratory of Ce1lular body structure and Metabolism, nationwide middle Institute, Bethesda, Maryland 3/8/63 l. a. Martin Kamen college of California San Diego, California 11/8/63 Chicago Joseph L. Rabinowitz Veterans management clinic Philadelphia, Pennsylvania once more, it's the editor's excitement to recognize the aid of his colleagues in making plans and operating some of the Symposia, in addition to the superb cooperation of the chairmen, who acquitted themselves of their appointed projects in a most productive demeanour, With humor and realizing. S. R. Boston, Mass.

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Extra info for Advances in Tracer Methodology: Volume 2 A collection of papers presented at the Sixth, Seventh, and Eight Symposia on Tracer Methodology plus other papers selected by the editor

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J. E .. in: Methods in Enzymology. Vol. III (S. P. Colowick and N. O. ). Academic Press. New York (1957). p. 397. 8. Rose. I. • Kellermeyer, R .. Stjernholrn. R.. and Wood. H. G .. J. Bioi. Chern. 237:3325 (1962). S. Abraham 59 9. Matthes, K. J .. Abraham. S.. and Chaikoff, I. L.. Biochim. Biophys. Acta 70:242 (1963). 10. , Ludowieg. H •• Normore. • Biochemistry 2:1009 (1963). 11. San Pietro, A.. J. BioI. Chern. 217:579 (1955). 12. Horecker, B. L .. , J. Bioi. Chern. 175:385 (1948). 13. K .. • J.

It is the purpose of the present report to mention some of the compounds that have been recently prepared by biological methods and to describe, in some detail, the synthesis of glucose-3,4-C14. The preparation of this hexose with C14 in carbons 3 and 4 by conventional organic chemical methods would, of course, be extremely difficult. METHODS MAKING USE OF PURIFIED ENZYME SYSTEMS Conversion of Glucose to Fructose by Organic and Biological Procedures One of the most successful methods for the conversion of isotopically-labeled glucose into isotopically-labeled fructose has been the procedure devised by Muntz and Carroll [1) in yields which exceed 70%.

34 Table XII. 2 B. E. Gordon and J. J. Madison 37 Table XIII. 47 Table XIV. 9 "/0 H replaced by D Methods for Labeling Compounds 38 Table XV. 7 0 1 2 3 4 5 6 7 8 9 10 11 Table XVI. Deuterium Distribution, Run 15-Dz/n-C 5 Number of D atoms per molecule 0 1 2 3 4 5 6 7 8 9 10 11 12 "/0 H replaced by D Hydrocarbon. "/om. 2 C14-LABELING USING CARBENE INSERTIONAPPLICATION TO SATURATED HYDROCARBONS * M. A. Muhs, E. L. Bastin, and B. E. , less use is made of them than would be expected. This is due chiefly to difficulties in obtaining radioactive tracers in their proper chemical forms.

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